Javascript bɛ baara la sisan i ka navigatɛri kɔnɔ.Nin siti in ka baarakɛcogo dɔw tɛna baara kɛ ni javascript dabilalen don.
Aw ye aw tɔgɔ sɛbɛn ni aw ka kunnafoni kɛrɛnkɛrɛnnenw ye ani fura kɛrɛnkɛrɛnnen min bɛ aw mago don aw la ani aw bɛna kunnafoni minnu di ni barokunw ye minnu bɛ an ka kunnafonidilanba kɔnɔ, an bɛna olu bɛn ɲɔgɔn ma ani ka PDF sɛbɛn ci aw ma o yɔrɔnin bɛɛ.
Ban-Lan-Gen granules bɛ dextran sodium sulfate (dextran sodium sulfate) kɔlɔlɔ basigilenw dɔgɔya sosow la, u kɛtɔ ka kɔnɔbara mikorobiyɔmu caman sɛmɛntiya ani ka banakɔtaa SCFA Derived-GLP-1 bɔli segin a cogo kɔrɔ la
Jiao Peng,1-3,*Li Xi,4,*Zheng Lin,3,5 Duan Lifang,1 Gao Zhengxian,2,5 Diehu,1 Li Jie,6 Li Xiaofeng,6 Shen Xiangchun,5 Xiao Haitao21Peking Iniwɛrisite Shenzhen dɔgɔtɔrɔso furakɛli ɲɛmɔgɔso, Shenzhen, Sinuwa jamana 2Shenzhen Iniwɛrisite kɛnɛyako dɔnniyaso furakɛli kalanso, Shenzhen, Sinuwa jamana; 3Guizhou Dɔgɔtɔrɔso Iniwɛrisite Ɛntɛrinɛti Teknoloji ɲininikɛyɔrɔ siya furakɛli ni Sinuwa laadala furakɛli yiriwali n’a waleyali Minisiri kalanko minisiriso, Guizhou mara furakɛli laboratuwari kunbaba, Guizhou Dɔgɔtɔrɔso Iniwɛrisite , Guiyang, Sinuwa jamana 4 Kɔnɔbarako ɲɛmɔgɔso, Pekin Iniwɛrisite Shenzhen dɔgɔtɔrɔso, Shenzhen, Sinuwa jamana; 5 Farmasi kalanso, Guizhou Dɔgɔtɔrɔso Iniwɛrisite, Etazini ka laboratuwari kunbaba min bɛ furakɛli jiriw baaracogo n’u kɛcogo kan, Guiyang; 6 Laboratowari furakɛli ɲɛmɔgɔso, Pekin Iniwɛrisite Shenzhen dɔgɔtɔrɔso, Shenzhen, Sinuwa [email protected] Shen Xiangchun, Farmasi kalanso, Guizhou Dɔgɔtɔrɔso Iniwɛrisite, Guizhou, Sinuwa jamana, 550004, Email [email protected] Laɲini: Furakɛli min sinsinnen bɛ GLP-1 kan, o ye banakɔtaa funu furakɛcogo kura ye.Ban-Lan-Gen (BLG) granules ye banakisɛfagalan TCM furakɛcogo dɔnta ye min bɛ se ka funu kɛlɛli baara jira funubana suguya caman furakɛli la.Nka, a ka banakisɛfagalanw nɔ min bɛ kɔnɔboli la ani a kɛcogo ma jɛya hali bi.FƐRƐW: Walasa ka dextran sodium sulfate (DSS)-induit chronic relapsing colitis sigi sen kan sosow la.Disease activity indices, histological markers ka jogin, ani sitokini proinflammatory hakɛw kɛra walasa ka BLG ka lakanani nɔ jateminɛ.BLG nɔ minnu bɛ kɔnɔbara mikorobiyɔmu ni kɔnɔbara la, olu kɛra GLP-1 hakɛ ye joli la ani Gcg, GPR41, ani GRP43 jirali ye kolotuguda la, kɔnɔbara mikorobiyɔmu hakɛ, SCFAs hakɛ min bɛ kɛ nɔgɔ la, ani GLP-1 bɔli ye soso kolotuguda epiteli fɔlɔ la cellules SCFA-derived GLP-1 production.Jaabiw: BLG furakɛli ye farikolo girinya dɔgɔyali, DAI, kolotuguda surunyali, kolotuguda farikolo tiɲɛni, ani TNF-α, IL-1β, ani IL-6 sitokini hakɛ min bɛ funu ɲɛ kolotuguda la, o dɔgɔya kosɛbɛ.Ka fara o kan, BLG furakɛli bɛ se ka kolotuguda Gcg, GPR41 ani GRP43 jirali ni joli GLP-1 hakɛ segin kosɛbɛ koloci sogow, ani ka dɔ fara SCFA dilanbagaw kan i n’a fɔ Akkermansia ani Prevotellaceae_UCG-001, ani ka banakisɛ caman dɔgɔya i n’a fɔ Eubacterium_xylanophilum_group, Ruminococcaceae_UCG-014, Intestinimonas ani Oscillibacter. Ka fara o kan, BLG furakɛli bɛ se ka dɔ fara SCFA hakɛ kan kosɛbɛ kɔnɔboli sogow ka nɔgɔ la. O waati kelen na, sɛgɛsɛgɛli minnu kɛra in vitro la, olu fana y’a jira ko soso minnu furakɛra ni BLG ye, olu ka nɔgɔ bɔlen bɛ se ka Murine kolosinsinnan misɛnnin fɔlɔw lawuli kosɛbɛ minnu bɛ GLP-1 bɔ. Kuncɛli : Nin sɔrɔlen ninnu b’a jira ko BLG bɛ se ka kɔnɔboli kɛlɛ. BLG bɛ se ka kɛ furakɛli ye, a dɔgɔyalenba la, a bɛ kɛ ni kɔnɔbara mikorobiyɔmu caman sɛgɛsɛgɛli ye ani ka banakɔtaa SCFA sɔrɔlen GLP-1 bɔli lasegin Fura layidu talenw kɔnɔboli basigilen kɔsegincogo la. Daɲɛ kolomaw : kɔnɔboli, Ban-Lan-Gen granules, kɔnɔbara mikorobiyɔmu, asidi tuluma cakɛda surunw, GLP-1
Kɔnɔnatumuw (UC) ye bana ye min bɛ mɛn kɔnɔbara ni kɔnɔbara la, a bɛ sɔrɔ kɔnɔboli segin-segin, kɔnɔdimi, farikolo girinya dɔgɔyali, ani banakɔtaa joli bɔlen kɔfɛ.1 Kɔsa in na, UC caya bɛ ka caya jamanaw la minnu tun ka dɔgɔn fɔlɔ, Sinuwa fana sen bɛ o la, ni tubabu jamanaw ka ɲɛnamaya kɛcogo bɛ ka caya ka taa a fɛ.2 O caya bɛ gɛlɛyabaw lase foroba ma kɛnɛyako la ani a bɛ nɔ jugu bila banabagatɔw ka baara seko la ani u ka ɲɛnamaya kɛcogo ɲuman na.Kɛrɛnkɛrɛnnenya la, UC banakisɛ sɔrɔcogo ma jɛya hali bi, nka a bɛ sɔn a ma ko jamu, sigida fɛnw, kɔnɔbara basigilenw, ani farikolo tangalanw bɛɛ bɛ kɛ sababu ye ka UC sɔrɔ.3 Hali sisan, fura tɛ UC la, wa furakɛli kuntilenna ye kɛnɛyaso la ka kɛnɛyaso taamasiɲɛw kɛlɛ, ka kɛnɛyaso taamasiɲɛw bila mɔgɔ la ani ka u mara remission, promote mucosal healing, and reduce recurrence.Furakɛcogo kɔrɔw ye aminosalicylates , corticosteroids, immunosuppressants, ani biologics ye.Nka, fura ninnu tɛ se ka nɔ ɲininen sɔrɔ ka da u kɔlɔlɔ suguya caman kan.4 Kɔsa in na, ko caman sɛgɛsɛgɛli y’a jira ko Sinuwaw ka tubabufura (TCM) ye sebaayaba jira ka dɛmɛ don ka UC lajɔ ni a baga ka dɔgɔn, o b’a jira ko TCM furakɛlicogo kura labɛnni ye furakɛli fɛɛrɛ ye min bɛ layidu ta UC.5-7
Banlangen Granules (BLG) ye Sinuwaw ka laadala fura ye min dilannen don ni Banlangen ju ji bɔlen ye.8 Ka fara a ka banakisɛfagalanw nafa kan, BLG bɛ se ka banakisɛfagalanw jira funubana suguya caman furakɛli la.9,10 Ka fara o kan, gulukosinɔlatiw (R,S-goitrin, progoitrin, epiprorubin ani glucoside bɔra u la ka bɔ u la Radix isatidis jilamaw) ani nukilisidiw (hypoxanthine, adenosine, uridine ani guanosine) ani indigo alkaloïdes i n’a fɔ indigo ni indirubine.11,12 Kalan tɛmɛnenw y’a jira ka ɲɛ ko adenosin, uridine ani indirubine faralen ɲɔgɔn kan, olu bɛ kɔlɔlɔw kɛlɛli fanga jira baganw ka misali suguya wɛrɛw la.13-17 Nka, a tɛ kɛ sɛgɛsɛgɛli minnu sinsinnen bɛ daliluw kan, olu kɛra walasa ka BLG nafa jateminɛ kɔnɔbara basigilen na.Nin kalan in kɔnɔ, an ye BLG ka lakanani nɔ sɛgɛsɛgɛ kɔnɔbara basigilen kɔseginlen na min bɛ sɔrɔ dextran sodium sulfate (DSS) fɛ C57BL/6 sogow la, ka a ye ko BLG tali da la, o ye DSS ka kolotuguda basigilen dɔgɔya kosɛbɛ sosow la Funu, a ɲɛnabɔli fɛɛrɛw bɛ tali kɛ kɔnɔbara mikorobiyɔmu caman sɛgɛsɛgɛli la ani kɔnɔbara la min bɛ sɔrɔ glucagon-like peptide-1 (GLP-1) bɔli la seginni na.
BLG granules (sukaro tɛ minnu na, NMPA sɔnna Z11020357; Beijing Tongrentang Technology Development Co., Ltd., Beijing, Sinuwa; kulu nimɔrɔ: 20110966) sanna furakɛlisow fɛ.DSS (Molecular Weight: 36.000–50.000 Daltons) sanna MP Biologicals fɛ (Santa Ana, USA).Sulfasalazine (SASP) (≥ 98% saniya), hematoxyline ani eosin sanna Sigma-Aldrich fɛ (St. Louis, MO, USA).Sogo TNF-α, IL-1β ani IL-6 luminex Elisa sɛgɛsɛgɛlikɛminɛnw sanna ka bɔ R&D systems (Minneapolis, MN, USA).Asidi asetiki, asidi propionic ani asidi butyric sanna Aladdin fɛ Industries (Shanghai, China).2-Asidi etilbutiririki sanna Merck KGaA fɛ (Darmstadt, Alimanjamana).
C57BL/6 sogo cɛman minnu si bɛ dɔgɔkun 6-8 la (u farikolo girinya ye garamu 18-22 ye) sanna ka bɔ Beijing Wetahe Laboratory Animal Technology Co., Ltd. (Beijing, China) fɛ, ka u bila sigida la min janya ye 22 ± 2 °C ye ni yeelen/ dibi cycle ye lɛrɛ 12 kɔnɔ.Sogow balola sogow ka dumuni nafama na ni ji minta ye fu dɔgɔkun kelen kɔnɔ walasa u ka se ka u yɛrɛ dege sigida kura la.The o kɔfɛ, sosow tilalen don kulu naani ye k’a sɔrɔ u ma kɛ cogo si la: kɔrɔsili kulu, DSS misali kulu, SASP furakɛli kulu (miligaramu 200/kg, da la) ani BLG furakɛli kulu (1 g/kg, da la).I n’a fɔ a jiralen bɛ cogo min na ja 1A kɔnɔ, ka kɛɲɛ ni an ka kalan tɛmɛnen ye, kɔnɔboli basigilen min bɛ segin ka kɛ k’a lajɛ, o kɛra sosow la ni DSS 1,8% siɲɛ saba ye tile 5 kɔnɔ, o kɔfɛ, ji dilanlen kɛra tile 7 kɔnɔ tile, ka kɛɲɛ ni an ka kalan tɛmɛnen ye.18 Sogo minnu tun bɛ SASP ni BLG furakɛli kuluw la, olu furakɛra ni SASP ni BLG ye, don o don k’a daminɛ tile 0 la.Ka kɛɲɛ ni kɔrɔbɔli fɔlɔw ye, BLG hakɛ tun sigilen don garamu 1/kg.O waati kelen na, SASP hakɛ tun sigilen don miligaramu 200/kg ka kɛɲɛ ni sɛbɛnw ye.4 Kɔlɔsili ni DSS modɛli kuluw ye o kelen sɔrɔ ji hakɛ min bɛ sɔrɔ kɔrɔbɔli bɛɛ kɔnɔ.
Jaa 1 BLG bɛ DSS-induite chronic relapsing colitis in mice.(A) Kɔnɔboli basigilen kɔseginlen ni furakɛli kɛcogo kɔrɔbɔli, (B) farikolo girinya caman yeli, (C) bana baarakɛcogo jiralan (DAI) hakɛ, (D) kolotuguda janya, (E) kolotuguda ja jiralen, (F) H&E staining Kolon (bonya, ×100) ani (G) histological score.Danaw bɛ jira iko cɛmancɛ ± SEM (n = 6).##p < 0,01 walima ###p < 0,001 vs control (Con) kulu; *p < 0,05 walima **p < 0,01 walima ***p < 0,001 ni DSS kulu ye.
Farikolo girinya, banakɔtaa kɛcogo, ani ɲɛgɛnɛsira joli bɔli sɛbɛnna don o don.Bana baarakɛcogo hakɛ (DAI) jateminɛna ni farikolo girinya hakɛ, banakɔtaa basigilen, ani kɔnɔbara basigilen hakɛw faralen ɲɔgɔn kan i n’a fɔ a ɲɛfɔra cogo min ka tɛmɛ.19 Kɔrɔbɔli laban na, sosow bɛɛ fagara ka u faga ani ka joli, nɔgɔ ani kɔnɔbara lajɛ walasa ka sɛgɛsɛgɛli wɛrɛw kɛ.
Kolotuguda yɔrɔ tun sirilen don foromalini na ka don parafin na.5-mikɔrɔn tilayɔrɔw kɛra ka u nɔgɔ ni ɛmatokisilini-ɛyosini (H&E) ye, o kɔfɛ, u fiyentɔya ani ka jate kɛ i n’a fɔ a ɲɛfɔra cogo min ka tɛmɛ.19
Kɔnɔbara basigilen ARN bɛɛ lajɛlen bɔra Trizol reagent fɛ (Invitrogen, Carlsbad, CA), o kɔfɛ ADNc bɔli kɛra ni reverse transcriptase ye (TaKaRa, Kusatsu, Shiga, Japon).PCR hakɛ kɛra ni PCR sitɛmu ye waati yɛrɛ la ni SYBR Green Master ye (Roche, Basel, Switzerland).Target gene transcripts were normalized to β-actin and data are analyzed baara kɛ ni 2-ΔΔCT fɛɛrɛ ye.Jɛnɛya daminɛcogo sinsinniw jiralen don jatebɔsen 1 na.
Sogo fɔlɔw ka kolosinsinnanw bɔli ni u ladonni kɛra i n’a fɔ a ɲɛfɔra cogo min ka tɛmɛ.20 Kuma surun na, sogo minnu si bɛ dɔgɔkun 6-8 la, olu kolotugudaw tigɛra fɔlɔ saraka kɔfɛ ni kɔnɔbara basigilen ye, o kɔfɛ, u dabɔra ka janya, ka u furakɛ ni Hanks Balanced Salt Solution (HBSS, ni kalisiyɔmu ni manje tɛ a la) ka u tigɛ ka kɛ milimɛtɛrɛ 0,5-1 ye minnu ka dɔgɔn pieces.O kɔfɛ, farikolo yɔrɔw sɔgɔra ni miligaramu 0,4/mL ye kolajɛnazi XI (Sigma, Poole, UK) la DMEM gansan na ka u bila santirifugare la 300 xg la miniti 5 kɔnɔ so funteni na.Aw bɛ furakisɛ in wuli kokura DMEM fɛnɲɛnamafagalan na (aw bɛ fara denso kɔnɔ misiden 10% kan, penisilini 100 Units/mL, ani 100 μg/mL streptomycine) 37 °C la ka tɛmɛ nilon mesh fɛ (pore size ~250 μm).Colonic epithelial cellules tila-tilalenw bilala galasi duguma minɛnw kɔnɔ ka u bila ni asidi asetiki, asidi propionic, asidi butyric, ani sosow ka nɔgɔ bɔlenw ye sanga 2 kɔnɔ 37°C la, 5% CO2.
Kolosinsinnanw kɛra kelen ye ni PBS ye, ani sitokiniw IL-6, TNF-α ani IL-1β hakɛ minnu bɛ kolotugudaw la, olu sɔrɔla ni luminex ELISA sɛgɛsɛgɛlikɛminɛnw ye (R&D systems, Minneapolis, MN, USA).O cogo kelen na, GLP-1 hakɛ minnu bɛ sosow ka kolosinsinnan fɔlɔw ka serum ni sɛnɛfɛnw sɛnɛyɔrɔ la, olu jateminɛna ni ELISA minɛn ye (Bioswamp, Wuhan, China) ka kɛɲɛ ni a dilabaga ka laadilikanw ye.
ADN bɛɛ lajɛlen bɔra nɔgɔ la ni ADN bɔli minɛn ye (Tiangen, Sinuwa).ADN jogo ni a hakɛ jateminɛna ni 260 nm/280 nm ani 260 nm/230 nm ye.O kɔfɛ, ka ADN bɔlen kelen-kelen bɛɛ kɛ misali ye, ni daminɛ kɛrɛnkɛrɛnnenw ye 338F (ACTCCTACGGGAGGCAGCAG) ani 806R (GGACTACHVGGGTWTCTAAT) were used to amplify the V3-V4 regions of the 16S rRNA gene in different regions.PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN, Germany), quantified by real-time PCR, and sequenced using the IlluminaMiseq PE300 sequencing platform (Illumina Inc., CA, USA).For bioinformatics analysis, data baarakɛcogo kɛra ka kɛɲɛ ni sariyaw ye minnu fɔra ka tɛmɛ.21,22 Kuma surun na, baara kɛ ni Cutadapt (V1.9.1) ye walasa ka raw express files filɛ.OTUw kɛra kulu ye ni UPARSE (version 7.0.1001) ye ni bɔɲɔgɔnko tigɛlen ye 97%, ani UCHIME kɛra ka chimeric sequences bɔ.Community composition analysis and classification kɛra ni RDP classifier ye (http://rdp.cme.msu.edu/) min sinsinnen bɛ SILVA ribosomal ARN jamu kunnafonidilan kan.
Asidi tuluma cakɛda surunw hakɛ (asidi asetiki, asidi propiyɔniki, ani asidi butiriki) hakɛ jateminɛna i n’a fɔ Tao ni a ɲɔgɔnnaw y’a ɲɛfɔ cogo min na ka tɛmɛ, ni fɛn dɔw sɛmɛntiyalen don.23 Kuma surun na, nɔgɔ miligaramu 100 fɔlɔla ka wuli ji mililitiri 0,4 na min ma ionize, o kɔfɛ asidi sulfuriki mililitiri 0,1 ani asidi sulfuriki mililitiri 0,5 Asidi 2-ethylbutyric (kɔnɔna sariya), o kɔ, a bɛ kɛ kelen ye ka a sumaya 4°C la. Aw bɛ centrifuge kɛ ni 12.000 rpm ye miniti 15 kɔnɔ C.A sanfɛji bɔra ni 0,5 mL ether ye ka pikiri kɛ GC kɔnɔ walasa ka sɛgɛsɛgɛli kɛ.Gazi chromatography (GC) sɛgɛsɛgɛli kama, sɛgɛsɛgɛli kɛra ni GC-2010 Plus gazi chromatograph (Shimadzu, Inc.) ye min bɛ ni flame ionization detector (FID) ye.Separation was achieved ni ZKAT-624 koloma ye, 30 m × 0,53 mm × 0,3 μm (Lanzhou Zhongke Antai Analytical Technology Co., Ltd., China).Kunnafoni sɔrɔla ni GC solution software ye (Shimadzu, Inc.).Tila hakɛ tun ye 10:1 ye, gazi tabaga tun ye azote ye, ani a jibɔ hakɛ tun ye 6 mL/min ye.Pikiri hakɛ tun ye 1 ye μL.Pikirikɛlan ni detektɛri funteni tun ye 300°C ye.Foro funteni tun bɛ to 140°C la miniti 13,5 kɔnɔ, o kɔfɛ, a bɛ bonya ka se 250°C ma ni 120°C/min ye funteni tun bɛ to miniti 5 kɔnɔ.
Kunnafoni bɛ jira iko cɛmancɛ ± hakɛ danmadɔ fili (SEM).Dɔnniya nafa jateminɛna ni ANOVA ye min bɛ kɛ sira kelen fɛ, o kɔfɛ Duncan ka sɛgɛsɛgɛli kɛra yɔrɔ caman na.GraphPad Prism 5.0 porogaramu (GraphPad Software Inc., San Diego, CA, USA) de kɛra jatebɔ bɛɛ la ani p < 0,05 jatera jateminɛ nafama ye.
A dɔnna kosɛbɛ ko UC ye kɔnɔbolibana basigilen ye min bɛ segin kɔ, kɔnɔdimi jugumanba, kɔnɔboli ani joli bɔli bɛ a la.O de kama, DSS-induite colitis chronic reconnaissance in sosow sigira sen kan walasa ka BLG ka kɔnɔboli kɛlɛli nafa jateminɛ (Ja 1A).Ni an ye a suma ni kɔrɔsili kulu ye, soso minnu bɛ DSS modɛli kulu la, olu farikolo girinya dɔgɔyara kosɛbɛ ani DAI ka ca, wa o fɛn caman b’a la ka wuli kosɛbɛ BLG furakɛli tile 24 kɔfɛ (Ja 1B ani C).Kɔnɔbara surunyali ye UC taamasiɲɛ nafama ye.I n’a fɔ a jiralen bɛ cogo min na ja 1D ni E la, soso minnu ye DSS sɔrɔ, olu ka kolo janya surunyara kosɛbɛ, nka u lafiyara BLG furakɛli fɛ.O kɔfɛ, histopathologique analyse kɛra ka assess colonic inflammation.H&E ja nɔgɔlenw ni banako jatew y’a jira ko DSS tali ye kolotuguda jɔcogo tiɲɛ kosɛbɛ, wa a kɛra sababu ye ka kripti tiɲɛni ni bana hakɛw dɔgɔya kosɛbɛ (Ja 1F ani G).Kɛrɛnkɛrɛnnenya la, BLG ka lakanani nɔ min bɛ kɛ ni a hakɛ ye garamu 1/Kg ye, o bɛ se ka suma ni SASP ta ye ni a tata hakɛ ye 200 ye mg/Kg.An bɛɛ lajɛlen na, nin sɔrɔlen ninnu b’a jira ko BLG bɛ se ka DSS ka kɔnɔboli basigilen kɔsegin juguya dɔgɔya sosow la.
TNF-α, IL-1β ani IL-6 ye kolotuguda funu taamasiɲɛ nafamaw ye.I n’a fɔ a jiralen bɛ cogo min na ja 2A na, DSS ye dɔ fara TNF-α, IL-1β ani IL-6 jamu jirali kan kolotuguda la ni i ye a suma ni kɔrɔsili kulu ye.BLG tali bɛ se ka nin fɛn caman wuli ka bɔ DSS fɛ.O kɔfɛ, an ye ELISA kɛ ka funu hakɛw dɔn sitokiniw TNF-α, IL-1β, ani IL-6 kolotuguda la.Jaabiw y’a jira fana ko TNF-α, IL-1β ani IL-6 hakɛ cayara kosɛbɛ kolotuguda la sosow la minnu furakɛra ni DSS ye, k’a sɔrɔ BLG furakɛli ye o cayaw nɔgɔya (Jaa 2B).
Jaa 2 BLG bɛ jeninida jirali ni sitokiniw bɔli bali funu ɲɛnama TNF-α, IL-1β ani IL-6 la sogo minnu furakɛra ni DSS ye, olu ka kolotuguda la.(A) TNF-α, IL-1β ani IL-6 kolotuguda jamu jirali (B) TNF-α, IL-1β ani IL-6 ka kolosinsinnan farikolojɔli dumuniw hakɛ.Kunnafoni bɛ jira i n’a fɔ ± SEM (n = 4–6).#p < 0.05 walima ##p < 0.01 walima ###p < 0.001 vs control (Con) kulu *p < 0,05 walima **p < 0,01 ni DSS kulu ye.
Banakɔtaa dɛsɛ nafa ka bon kosɛbɛ UC banakisɛ sɔrɔcogo la.24 Walasa ka sɛgɛsɛgɛli kɛ ni BLG bɛ sogo minnu furakɛra ni DSS ye, olu ka kɔnɔbara mikorobiyɔmu sɛgɛsɛgɛ, 16S rRNA sinsinni kɛra walasa ka kɔnɔbara kɔnɔfɛnw banakisɛ jɛkulu sɛgɛsɛgɛ.Venn ja b’a jira ko kulu saba ninnu bɛ OTU 385 kɛ ɲɔgɔn fɛ.Waati kelen na, kulu kelen-kelen bɛɛ tun bɛ ni OTU kɛrɛnkɛrɛnnenw ye (Jiracogo 3A).Ka fara o kan, Chao1 index ni Shannon index min jiralen don ja 3B ni C la, o y’a jira ko kɔnɔbara mikorobiyɔmu sigida suguya caman dɔgɔyara sosow la minnu furakɛra ni BLG ye, bawo Shannon index dɔgɔyara kosɛbɛ BLG furakɛli kulu la.Principal component analysis (PCA) ani principal coordinate analysis (PCoA) kɛra sababu ye ka kulu saba ni ɲɔgɔn cɛ kuluw sigicogo dɔn y’a jira ko sogo minnu furakɛra ni DSS ye, olu ka sigida labɛncogo farala ɲɔgɔn kan ka jɛya BLG furakɛli kɔfɛ (Ja 3D ani E).Nin kunnafoniw b’a jira ko BLG furakɛli ye nɔba bila sosow ka sigida labɛncogo la minnu bɛ ni DSS ye.
Jaa 3 BLG bɛ kɔnɔbara mikorobiyɔmu suguya caman Changer sosow la minnu bɛ ni DSS-induite colitis ye.(A) Venn diagram of OTU, (B) Chao1 index, (C) Shannon’s richness index, (D) Principal Component Analysis (PCA) score plot of OTU, (E) OTU Principal Coordinate Analysis (PCoA) score Jaa. Kunnafoni bɛ jira i n’a fɔ cɛmancɛ ± SEM (n = 6).**p < 0.01 vs DSS kulu.
Walasa ka fɛn kɛrɛnkɛrɛnnenw jateminɛ fɛnɲɛnɛma mikorobiyɔtiw la, an ye kɔnɔbara mikorobiyɔmuw danfara sɛgɛsɛgɛ taxonomi hakɛ bɛɛ la.I n’a fɔ a jiralen bɛ cogo min na ja 4A kɔnɔ, phyla kunbaba minnu tun bɛ kulu bɛɛ kɔnɔ, olu tun ye Firmicutes ni Bacteroidetes ye, o kɔfɛ Verrucomicrobia.Firmicutes ni Firmicutes/Bacteroidetes hakɛw cayalenba cayara kosɛbɛ san kɔnɔ sogo minnu furakɛra ni DSS ye, olu ka nɔgɔ sigidaw ni u ni kɔrɔsili sogow ta kɛra ɲɔgɔn fɛ, wa o fɛn caman wulila ka segin kɔ kosɛbɛ BLG furakɛli kɔfɛ .Kɛrɛnkɛrɛnnenya la, BLG furakɛli ye dɔ fara kosɛbɛ Verrucobacterium caya kan kosɛbɛ sosow ka nɔgɔ la minnu bɛ ni DSS ye, kɔnɔboli bɛ minnu na.Du kɔnɔ, nɔgɔ sigidaw tun bɛ Lachnospiriaceae fɛ, Muribaculaceae, Akkermansiaceae, Ruminococcaceae ani Prevotellaceae (Ja 4B).Ni an y’a suma ni DSS kulu ye, BLG dɔgɔyali ye dɔ fara Akkermansiaceae caya kan, nka a ye dɔ bɔ Lachnospiraceae ni Ruminococcaceae caya la.Kɛrɛnkɛrɛnnenya la, suguya hakɛ la, nɔgɔ mikrobiyɔta tun bɛ minɛ by Lachnospira_NK4A136_group, Akkermansia and Prevotellaceae_UCG-001 (Ja 4C).Nin sɔrɔ in y’a jira fana ko BLG furakɛli ye mikrobiyɔmu balanbaliya kɔsegin ka ɲɛ DSS gɛlɛya jaabi la, o min bɛ sɔrɔ Eubacterium_xylanophilum_group, Ruminococcaceae_UCG-014, Intestinimonas ani Oscillibacter, ani dɔ farali Akkermansia ni Prevotellaceae_UCG-001 kan.
Jaa 4 BLG bɛ kɔnɔbara mikorobiyɔmu caya caman sɛmɛntiya DSS-induite colitis sogow la.(A) Kɔnɔnatumu mikorobiyɔmu caya phylum hakɛ la; (B) Kɔnɔnatumu mikorobiyɔmu caya denbaya kɔnɔ; (C) Kɔnɔnatumuw ka mikorobiyɔmu caya suguya la.Kunnafoni bɛ jira iko cɛmancɛ ± SEM (n = 6).#p < 0.05 walima ###p < 0.001 vs control (Con) kulu *p < 0,05 walima **p < 0,01 walima ***p < 0,001 ni DSS kulu ye.
Ni an y’a jateminɛ ko asidi tuluma cakɛda surunw (SCFA) ye Akkermansia ni Prevotellaceae_UCG-001 farikolojɔlan kunbabaw ye, k’a sɔrɔ asetati, poropiyɔnati ani butirati ye SCFAw ye minnu ka ca banakɔtaa la, 25-27 an bɛ an ka kalan na hali bi.I n’a fɔ a jiralen bɛ cogo min na ja 5 na, nɔgɔ asetati, poropiyɔnati, ani butirati hakɛ tun bɛ dɔgɔyara kosɛbɛ DSS furakɛli kulu la, ka sɔrɔ BLG furakɛli bɛ se ka o dɔgɔyali in bali kosɛbɛ.
Jaa 5. BLG bɛ dɔ fara SCFA hakɛ kan sosow ka nɔgɔ la minnu bɛ ni DSS ye.(A) Asidi asetiki hakɛ min bɛ nɔgɔ la; (B) asidi propiyɔni hakɛ min bɛ nɔgɔ la; (C) asidi butiriki hakɛ min bɛ nɔgɔ la.Kunnafoni bɛ jira i n’a fɔ cɛmancɛ ± SEM (n = 6).#p < 0.05 walima ##p < 0.01 vs control (Con) kulu; *p < 0,05 walima **p < 0,01 ni DSS kulu ye.
An ye Pearson ka jɛɲɔgɔnya coefficient jate ka t’a fɛ SCFA diferential genus-level differential SCFA ni fecal microbiota cɛ.I n’a fɔ a jiralen bɛ cogo min na ja 6 na, Akkermansia ni asidi propionic (Pearson = 0,4866) ani asidi butyric (Pearson = 0,6192) sɔrɔli bɛ tali kɛ ɲɔgɔn na cogo ɲuman na.O ni Enteromonas ni Oscillobacter fila bɛɛ ni asetate tɛ kelen ye sɔrɔli, ni Pearson ka coefficients ye 0,4709 ani 0,5104 ye.O cogo kelen na, Ruminococcaceae_UCG-014 ni asidi propionic (Pearson = 0,4508) ani asidi butyric (Pearson = 0,5842) sɔrɔli bɛ tali kɛ ɲɔgɔn na cogo jugu la.
Jaa 6 Pearson ka jɛɲɔgɔnya sɛgɛsɛgɛli SCFA danfaralenw ni kolotuguda banakisɛw cɛ.(A) Enteromonas ni asidi asetiki; (B) Basilisi min bɛ kɛ ni asidi asetiki ye; (C) Akkermansia ni asidi propiyɔni; (D) Ruminococcus_UCG-014 ni asidi poropiyɔniki; (E) Akkermansia ni asidi butiriki; (F) ) Ruminococcus _UCG-014 ni asidi butiriki ye.
Glucagon-like peptide-1 (GLP-1) ye proglucagon (Gcg) ka bamanankan-falen kɔfɛ fɛn ye min bɛ se ka kɛ selilɛri suguya kɛrɛnkɛrɛnnen ye, ni a bɛ se ka banakisɛfagalanw kɛlɛ.28 I n’a fɔ a jiralen bɛ cogo min na ja 7 na, DSS ye dɔ bɔ kosɛbɛ Gcg mRNA jirali la.Kɔlɔn ni BLG furakɛli bɛ se ka DSS ka Gcg dɔgɔyali kɔsegin kosɛbɛ ni a ni kɔrɔsili kulu ta kɛra ɲɔgɔn fɛ (Jaa. 7A).O waati kelen na, GLP-1 hakɛ min bɛ joli la, o dɔgɔyara kosɛbɛ DSS furakɛli kulu la, wa BLG furakɛli tun bɛ se ka o dɔgɔyali bali kosɛbɛ (Ja 7B).Ikomi asidi tuluma cakɛda surunw bɛ se ka GLP-1 bɔli lawuli G-protéine-couplé receptor 43 (GRP43) ani G-protein-coupled receptor 41 (GRP41) activation fɛ, an fana ye GPR41 ni GRP43 sɛgɛsɛgɛ kolotuguda sogow ka kolotuguda la, k’a ye ko GRP43 ni GPR41 kolotuguda mRNA jirali dɔgɔyara kosɛbɛ DSS gɛlɛya kɔfɛ, wa BLG furakɛli bɛ se ka o dɔgɔyali ninnu kisi ka ɲɛ (Jaa 7C ni D).
Jaa 7 BLG bɛ dɔ fara GLP-1 hakɛ kan joli la ani kolotuguda Gcg, GPR41 ani GRP43 mRNA jirali sogow la minnu furakɛra ni DSS ye.(A) Gcg mRNA jirali kolotuguda la (B) GLP-1 hakɛ min bɛ joli la; (C) GPR41 mRNA jirali kolotuguda la; (D) GPR43 mRNA jirali kolotuguda la.Kunnafoni bɛ jira iko cɛmancɛ ± SEM (n = 5–6).#p < 0.05 walima ##p < 0.01 vs control (Con) kulu; *p < 0,05 ni DSS kulu ye.
Ikomi BLG furakɛli bɛ se ka dɔ fara GLP-1 hakɛ kan joli la, ka Gcg mRNA jirali kɛ kolotuguda la, ani ka SCFA hakɛ caya nɔgɔ la sosow la minnu furakɛra ni DSS ye, an ye asetati, poropiyɔnati ani butirati sɛgɛsɛgɛ ka taa a fɛ ani fana ka bɔ sosow la minnu bɛ furakɛ ni kɔrɔsili (F-Con), DSS koloci (F-Con) -DSS) ani sogo minnu furakɛra ni BLG ye (F-BLG) GLP-1 bɔli la soso fɔlɔw la kolosinsinnanw.I n’a fɔ a jiralen bɛ cogo min na ja 8A na, sosow ka kolosinsinnanw fɔlɔ minnu furakɛra ni asidi asetiki, asidi propiyɔniki ani asidi butiriki 2 mM ye, olu ye GLP-1 bɔli lawuli kosɛbɛ, o bɛ bɛn kalan tɛmɛnenw ma.29,30 O cogo kelen na, F-Con, F-DSS, ani F-BLG bɛɛ (o bɛ bɛn nɔgɔ g 0,25 ma) a ye GLP-1 bɔli lawuli kosɛbɛ ka bɔ sosow ka kolotuguda selilɛri fɔlɔw la.Kɛrɛnkɛrɛnnenya la, GLP-1 min bɔra sosow ka kolotuguda selilɛri fɔlɔw fɛ minnu furakɛra ni F-DSS ye, o hakɛ tun ka dɔgɔ kosɛbɛ ka tɛmɛ sosow ka kolotuguda selilɛri fɔlɔw kan minnu furakɛra ni F-DSS ye.(Ja 8B).Nin kunnafoniw b’a jira ko BLG furakɛli seginna kosɛbɛ banakɔtaa SCFA-sɔrɔlen GLP-1 sɔrɔli.
Jaa 8 SCFA min bɛ bɔ BLG la, o bɛ GLP-1 bɔli lawuli ka bɔ sosow ka kolotuguda selilɛri fɔlɔw la.(A) Asidi asetiki, asidi propiyɔniki ani asidi butiriki ye GLP-1 bɔli lawuli ka bɔ sosow ka kolotuguda selilɛri fɔlɔw la (B) nɔgɔ bɔlenw F-Con, F-DSS ani F-BLG ye sosow ka kolosinsinnan fɔlɔw lawuli GLP-1 hakɛ min bɔra.Kɔnɔbara basigilen selilu tilayɔrɔw bilala galasi duguma petiri minɛnw kɔnɔ ka u furakɛ ni asidi asetiki 2 mM ye, asidi propiyɔniki, asidi butiriki, ani nɔgɔ bɔlenw F-Con, F-DSS, ani F-BLG (a bɛ bɛn nɔgɔ garamu 0,25 ma), o cogo kelen na. Lɛrɛ 2 37°C la, CO2 5%, o cogo kelen na.GLP-1 hakɛ min bɔra sosow ka kolotuguda ɲɛgɛnɛsira fɔlɔw la, o sɔrɔla ELISA fɛ.Kunnafoni bɛ jira iko cɛmancɛ ± SEM (n = 3).#p < 0.05 walima ##p < 0.01 ni blank walima F-Con ye *p < 0,05 ni F-DSS ye.
Kuma surunw: Ace, asidi asetiki; Pro, asidi propiyɔni; nka, asidi butiriki; F-Con, nɔgɔ bɔlen min bɛ bɔ sosow la minnu bɛ kɔrɔsi; F-DSS, o ye nɔgɔ bɔlen ye ka bɔ kɔnɔboli sogow la; F-BLG, ka bɔ kolotuguda furakɛli la ni BLG ye Sogo funulenw ka nɔgɔ bɔlenw.
Diɲɛ kɛnɛyatɔnba y’a jate bana ye min tɛ se ka bana kɛlɛ, UC bɛ ka kɛ farati ye diɲɛ kɔnɔ; nka, fɛɛrɛ ɲumanw minnu bɛ se ka kɛ ka bana in fɔ ka ɲɛ, k’a kunbɛn, ani k’a furakɛ, olu dan ye hali bi.O de kama, kɔrɔtɔko ka kan ka furakɛli fɛɛrɛ kuraw sɛgɛsɛgɛ ani k’u labɛn minnu tɛ mɔgɔ tɔɔrɔ ani minnu bɛ se ka kɛ UC la.Sinuwaw ka laadala furaw ye sugandi ye min bɛ layidu ta bawo a jirala ko Sinuwaw ka laadala fura labɛncogo caman bɛ se ka UC furakɛ Sinuwa jamanadenw na san kɛmɛ caman kɔnɔ, wa u bɛɛ ye fɛnɲɛnamaw ye minnu bɛ bɔ fɛnɲɛnamaw la ani minnu bɛ bɔ fɛnɲɛnamaw la minnu fanba tɛ kojugu ye hadamadenw ni baganw ma.31,32 Nin kalan in kun tun ye ka Sinuwaw ka laadala fura labɛncogo ɲini min tɛ mɔgɔ tɔɔrɔ ani min bɛ se ka kɛ UC furakɛli kama ani ka a kɛcogo sɛgɛsɛgɛ.BLG ye Sinuwaw ka furabulu lakodɔnnen ye min bɛ kɛ ka mura furakɛ.8,33 Baara kɛlen an ka laboratuwari kɔnɔ ani mɔgɔ wɛrɛw la, o y’a jira ko indigo, n’o ye Sinuwa laadala fura dilannen ye min dilannen don ni BLG ye, b’a jira ko nafa caman bɛ UC furakɛli la hadamadenw ni baganw na.4,34 Nka, BLG ka kɔnɔboli kɛlɛli nɔw n’a nɔfɛkow A kɛcogo ma jɛya.Sin kalan in kɔnɔ, an ka jaabiw b’a jira ko BLG bɛ DSS ka kolotuguda funu dɔgɔya kosɛbɛ, o min bɛ tali kɛ kɔnɔbara mikorobiyɔmu caman cili la ani GLP-1 min bɛ sɔrɔ kɔnɔbara la, o seginni na.
A dɔnna kosɛbɛ ko UC bɛ taamasiɲɛ kɛ ni waati seginni ye ni kɛnɛyaso taamasiɲɛw ye minnu bɛ kɛ cogoya la, i n’a fɔ farikolo girinya dɔgɔyali, kɔnɔboli, joli bɔli kɔkolo la, ani kolotuguda ɲɛgɛnɛsira tiɲɛni caman.35 O la, kɔnɔboli basigilen min bɛ segin ka kɛ, o kɛra ni DSS 1,8% kɛli ye siɲɛ saba tile duuru kɔnɔ, o kɔfɛ, ji minni kɛra tile wolonwula kɔnɔ.I n’a fɔ a jiralen bɛ cogo min na ja 1B kɔnɔ, i n’a fɔ a jiralen bɛ cogo min na ja 1B la, kɔnɔboli, kɔnɔboli jugumanba. girinya jiginni jiginni ni DAI jatew y’a jira ko kɔnɔboli basigilen min bɛ segin-ka-bɔ, olu daminɛna ka ɲɛ.Sogo minnu bɛ kulu min furakɛra ni BLG ye, olu ye u kɛnɛyali jira ka bɔ tile 8 la, o min ni tile 24 tɛ kelen ye kosɛbɛ.Yɛlɛma kelenw fana kɔlɔsira DAI hakɛ la, o b’a jira ko kolotuguda joginni ni funu cogoya fana ɲɛnabɔra.Kɔnɔbara joginni ni funu cogoya siratigɛ la, janya, kolotugudaw tiɲɛni, ani jeninida jirali ni sitokiniw minnu bɛ banakisɛfagalanw kɛ TNF-α, IL-1β, ani IL-6 la kolotuguda la, olu fana ɲɛnabɔra kosɛbɛ BLG furakɛli kɔfɛ.A bɛɛ lajɛlen na, nin jaabi ninnu b’a jira ka jɛya ko BLG bɛ se ka kɛ kɔnɔboli basigilen furakɛli la sosow la.
BLG b’a ka furakɛli nɔw bila cogo di?Sɛgɛsɛgɛli tɛmɛnen caman y’a jira ko kɔnɔbara basigilenw jɔyɔrɔ ka bon UC banakisɛ sɔrɔli la, wa furakɛli minnu sinsinnen bɛ mikrobiyɔmu kan ani minnu ɲɛsinnen bɛ mikrobiyɔmu ma, olu bɔra kɛnɛ kan ka kɛ fɛɛrɛ ye min bɛ mɔgɔ sama kosɛbɛ UC furakɛli la.Nin kalan in kɔnɔ, an y’a jira ko BLG furakɛli kɛra sababu ye ka fɛn caman Changements dans la microbiota de UC BLG ka lakanani nɔ min bɛ sɔrɔ DSS fɛ kɔnɔboli la, o bɛ tali kɛ kɔnɔbara mikorobiyɔmu caman sɛgɛsɛgɛli la.Nin kɔlɔsili in bɛ bɛn hakilina ma ko kɔnɔbara mikorobiyɔmu ka homɛostasi porogaramu seginni ye fɛɛrɛ nafama ye walasa ka TCM labɛnw nafa faamuya.36,37 Kɛrɛnkɛrɛnnenya la, Akkermansia ye banakisɛ ye min tɛ Gram-negatif ye ani min tɛ fiɲɛ bila kosɛbɛ, min bɛ balo nɔgɔ la kɔnɔbara lamini, min bɛ mucins tiɲɛ, ka asidi propionic bɔ, ka goblet cellules danfara lawuli, ani ka mucosa mara. function of barrier integrity.26 Dɔgɔtɔrɔso ni baganw ka kunnafoniw caman b’a jira ko Akkermansia ni ɲɛgɛnɛsira kɛnɛmanw bɛ tali kɛ ɲɔgɔn na kosɛbɛ,38 ani Akkermansia spp. bɛ se ka ɲɛgɛnɛsira funu ɲɛ kosɛbɛ.39 An ka sisan kunnafoniw b’a jira ko Akkermansia caya hakɛ bɛ caya kosɛbɛ BLG furakɛli kɔfɛ.Ka fara o kan, Prevotellaceae_UCG-001 ye banakisɛ ye min bɛ SCFA dilan.27 Kalan caman y’a jira ko Prevotellaceae_UCG-001 sɔrɔla a caya hakɛ dɔgɔyalen na baganw nɔgɔ la minnu bɛ ni colitis.40,41 An ka sisan kunnafoniw fana b’a jira ko BLG furakɛli bɛ se ka dɔ fara Prevotellaceae_UCG-001 caya kan kosɛbɛ sogo minnu furakɛra ni DSS ye a ni IL-6 ni IL-1β hakɛw ni bana hakɛw bɛ tali kɛ ɲɔgɔn na cogo ɲuman na.43,44 Kɛrɛnkɛrɛnnenya la, BLG furakɛli ye dɔ bɔ kosɛbɛ Oscillibacter caya la soso minnu furakɛra ni DSS ye, olu ka nɔgɔ la.Kɛrɛnkɛrɛnnenya la, nin banakisɛ minnu BLG caman b’u la, olu de tun ye banakisɛw ye minnu bɛ SCFA sɔrɔ kosɛbɛ.Kalan caman kɛra ka tɛmɛ, olu y’a jira ko SCFAw bɛ se ka nafa caman lase kɔnɔbara la inflammation and protection of intestinal epithelial integrity.45,46 An ka sisan kunnafoniw y’a jira fana ko SCFA asetati, poropiyoni ani butirati hakɛ cayara kosɛbɛ DSS furakɛli kɛlenw na sosow la.Ni u farala ɲɔgɔn kan, nin sɔrɔlen ninnu b’a jira ka jɛya ko BLG furakɛli bɛ se ka DSS-induite SCFA-producing banakisɛw bonya kosɛbɛ sosow la minnu ka bana basigilen don kɔnɔboli min bɛ segin ka kɛ.
GLP-1 ye inkɛrɛtini ye min bɛ sɔrɔ kosɛbɛ ɲɛgɛnɛsira ni kɔnɔbara la, wa a jɔyɔrɔ ka bon kɔnɔbara lankolonya bilali kɔfɛ ani ka joli sukaro dɔgɔya dumuni kɔfɛ.47 Dalilu b’a jira ko dipeptidil peptidase (DPP)-4, GLP-1 minɛbaga agoniste, ani GLP-1 nanomedicine bɛ se ka banakɔtaa funu nɔgɔya kosɛbɛ sosow la.48-51 I n’a fɔ a fɔra cogo min na ɲinini tɛmɛnenw na, SCFA hakɛ caman sɔrɔli tun bɛ tali kɛ GLP-1 hakɛ la joli la hadamadenw ni sosow la. 52 An ka kunnafoniw b’a jira sisan ko BLG furakɛli kɔfɛ, joli la GLP-1 hakɛ ni Gcg mRNA jirali cayara kosɛbɛ.O cogo kelen na, GLP-1 bɔli cayara kosɛbɛ kolotuguda sɛnɛfɛnw na, ka tugu lawulili kɔ ni nɔgɔ bɔlenw ye minnu bɔra kɔnɔboli sogow la minnu furakɛra ni BLG ye.SCFAw bɛ nɔ bila u bɔli la cogo di GLP-1?Gwen Tolhurst ni a ɲɔgɔnnaw. a y’a jira ko SCFA bɛ se ka GLP-1 bɔli lawuli GRP43 ni GPR41 fɛ.29 An ka sisan kunnafoniw fana b’a jira ko BLG furakɛli bɛ dɔ fara kosɛbɛ GRP43 ni GPR41 ka ARNm jirali kan sosow kɔnɔ minnu furakɛra ni DSS ye.O kunnafoniw b’a jira ko BLG furakɛli bɛ se ka GLP-1 bɔli lasegin SCFA fɛ ni GRP43 ni GPR41 ye.
BLG ye fura ye min bɛ mɛn ka sɔrɔ dɔgɔtɔrɔso la (OTC) Sinuwa jamana na. BLG hakɛ min bɛ se ka muɲu Kunming sogow la, o ye garamu 80/Kg ye, wa baga jugumanba si ma ye.53 Sisan, BLG hakɛ min fɔra (ni sukaro tɛ) hadamadenw na, o ye garamu 9-15 ye tile kɔnɔ (siɲɛ 3 tile kɔnɔ).An ka sɛgɛsɛgɛli y’a jira ko BLG ni kilogaramu 1/Kg ye kɔnɔboli basigilen kɔsegin DSS fɛ sosow.Nin hakɛ in bɛ gɛrɛ BLG hakɛ la min bɛ kɛ kɛnɛyaso la.An ka sɛgɛsɛgɛli y’a jira fana ko a kɛcogo bɛ kɛ sababu ye, a dɔgɔyalenba la, fɛn caman caman cili fɛ kɔnɔbara basigilen na, kɛrɛnkɛrɛnnenya la SCFA bɔli banakisɛw la, i n’a fɔ Akkermansia ani Prevotellaceae_UCG-001, walasa ka GLP-1 bɔli segin kɔnɔbara la.Nin sɔrɔlen ninnu b’a jira ko BLG ka kan ni fɛn wɛrɛw ye jateminɛ kɛra furakɛli fɛɛrɛ ye min bɛ se ka kɛ kɔnɔboli furakɛli la kɛnɛyaso la.Nka, a bɛ kɔnɔbara mikorobiyɔmu sɛgɛsɛgɛ cogo tigitigi min fɛ, o bɛ to ka tiɲɛ ni sosow ye minnu tɛ mikrobiyɔmu sɔrɔ ani nɔgɔ banakisɛw cili fɛ.
Ace, asidi asetiki; nka, asidi butiriki; BLG, pandan ye; DSS, dekstran sulfate de sodium; DAI, banaw baarakɛcogo jiralan; DPP, dipɛtidili pepitidazi; FID, tasuma ionizasiyɔn detektɛri; F-Con, control Sosow ka nɔgɔ bɔlenw; F-DSS, DSS kɔnɔboli sogow ka nɔgɔ bɔlenw; F-BLG, o ye kɔnɔboli sogo minnu furakɛra ni BLG ye, olu ka nɔgɔ bɔlenw; GLP-1, pepitiri-1 min bɛ i n’a fɔ glukagon; Gcg, gulukagɔni, gulukagɔni; gazi sɛgɛsɛgɛli, gazi sɛgɛsɛgɛli; GRP43, G poroteyini ni ɲɔgɔn cɛ minɛbaga 43; GRP41, G protéine-couplé récepteur 41; H&E, ɛmatokisilini-ɛyosini; HBSS, Hanks ka kɔgɔjibɔlan balansi ; OTC, OTC, OTC ye; PCA, o ye yɔrɔ kolomaw sɛgɛsɛgɛli ye; PCoA, n’o ye koordinatɛri sɛgɛsɛgɛli kunbaba ye; Pro, asidi propiyɔni; SASP, sulfasalazini, sulfasalazini; SCFA, asidi tuluma cakɛda surunw; Sinuwaw ka furakɛli, Sinuwaw ka laadala furakɛli; UC, kɔnɔbara basigilen.
Kɔrɔbɔli sariyaw bɛɛ sɔnna Pekin Iniwɛrisite ka baganw ka jogoɲumanya jɛkulu fɛ Shenzhen-Hong Kong Iniwɛrisite ka dɔnniya ni seko ni dɔnko dɔgɔtɔrɔso (Shenzhen, Sinuwa) ka kɛɲɛ ni sigida ka laadilikanw ni baganw ka sariyaw ye (jogoɲumanya nimɔrɔ A2020157).
Sɛbɛnnikɛlaw bɛɛ ye dɛmɛba don hakilinata ni u dilanni na, kunnafonidilanw sɔrɔli la, walima kunnafonidilanw sɛgɛsɛgɛli ni u kɔrɔfɔli la; a sen donna barokun sɛbɛnni na walima ka hakililata nafamaw lajɛ kosɛbɛ; bɛnna a kan ka bololasɛbɛn in bila gafe in kɔnɔ min bɛ sen na sisan ; a laban na, a sɔnna o bɔko ma walasa a ka bɔ kɛnɛ kan; A bɛ baara fan bɛɛ ɲɛnabɔ.
Nin baara in dɛmɛna ni Sinuwa jamana ka nafolosɔrɔko tɔnba ye (81560676 ani 81660479), Shenzhen Iniwɛrisite ka porozɛ fɔlɔ (86000000210), Shenzhen dɔnniya ni seko ni dɔnko kokuradon jɛkulu ka nafolo (JCYJ20210324093810026), ani Guangdong mara furakɛli dɔnniya ni seko ni dɔnko ɲinini nafolo (A2020157 ani A2020272), Guizhou Dɔgɔtɔrɔso Iniwɛrisite furakɛlitɔn Guizhou mara la, wari bɛ bɔ Key Laboratory (YWZJ2020-01) ani Peking Iniwɛrisite Shenzhen dɔgɔtɔrɔso (JCYJ2018009) fɛ.
1. Tang B, Zhu J, Zhang B, et al.Tripitolidi ka furakɛli seko i n’a fɔ banakisɛfagalan dextran sodium sulfate-iduced experimental colitis la sosow la.pre-immune.2020;11:592084.doi: 10.3389/fimmu.2020.592084
2. Kaplan GG.IBD doni diɲɛ kɔnɔ: k’a ta san 2015 la ka se san 2025 ma.Nat Rev Gastroenterol Hepatol.2015;12:720–727.doi: 10.1038/nrgastro.2015.150
3. Peng J, Zheng TT, Li Xue, et al.Alkaloïdes minnu bɛ sɔrɔ jiriw la: bana caman sɛmɛntiyalanw layidu ta banakɔtaa funubana na.Prepharmacology.2019;10:351.doi:10.3389/fphar.2019.00351
4. Xiao Haiteng, Peng Jie, Wen B, et al.Indigo Naturalis bɛ kolotuguda oksidan degun ni Th1/Th17 jaabiw bali DSS-induite colitis la sosow la.Oxid Med Cell Longev.2019;2019:9480945.doi: 10.1155/2019/9480945
5. Chen M, Ding Y, Tong Z. Sophora flavescens (Sophora flavescens) Sinuwaw ka furabuluw nafa ni a lakanani furakɛli la: kɛnɛyaso daliluw ani a kɛcogo minnu bɛ se ka kɛ.Prepharmacology.2020;11:603476.doi:10.3389/fphar.2020.603476
6. Cao Fang, Liu Jie, Sha Benxing, Pan HF.Sɔrɔfɛnw: fura minnu bɛ se ka kɛ ka banakɔtaa funu furakɛ.Curr Pharmaceuticals.2019;25:4893–4913.doi: 10.2174/1381612825666191216154224
7. Zhang C, Jiang M, Lu A. Hakilila minnu bɛ kɛ kɔnɔbara basigilen furakɛli dɛmɛnan kan ni Sinuwaw ka laadala furaw ye.Clinical Rev Allergy Immunization.2013;44:274–283.doi: 10.1007/s12016-012-8328-9
8. Li Zhongteng, Li Li, Chen TT, et al.Banlangen granules nafa ni u lakanani waati kɔnɔ kunfilanitu furakɛli la: kalan kɛcogo dɔ min bɛ kɛ ka ɲɛsin sɛgɛsɛgɛli ma min kɛra ni mɔgɔw ye.trial.2015;16:126.doi: 10.1186/s13063-015-0645-x